TABLE 2.

Functions of pXO1 ORF products

ORF	Length of product (amino acids)	Locationa	Function		Unique
			Putative (this study)	Determined by Okinaka et alb
1	167	S/M
2	192	M	MDR permeasec
3	211	S/M			+
4	209	C	Repeat motif protein bdr7ac
5	106	C
6	166	C
7	602	S/M	Reverse transcriptase	Reverse transcriptase
8	861	C	Helicasec
9	75	S/M
10	363	S/M	Type I restriction enzyme homolog, methyltransferase
11	117	C	Repeat proteinc		+
12	230	S/M
13d	1,320	S/M	Sir2 + regulatory protein	Rhoptry protein
14d	564	C	Clp ATPase, LacIc
15	80	C
16d	572	C	Bacterial transfer complexc
17	47	S/M
18	315	C	Integrase	Integrase, recombinase
19	73	C			+
20	498	C	Repeat proteinc
21	81	C
22	91	C	Adenine nucleotide hydrolase fragmentc
23	461	C	IepA truncated	Maturase-related protein
24	132	C	Nucleotidyl transferase
25	57	C
26	64	C			+
27	94	C			+
28	87	C			+
29	274	S/M	ATPase DNA repairc
30	155	C	Cystatin-likec		+
31	198	C			+
32	327	S/M	Ribosomal protein SIc
33	259	S/M	Spectinomycin 3′ adenyltransferase
34	68	S/M	Transcriptional regulator
35	478	S/M	Transposase	Transposase
36	484	S/M	Transposase	Transposase
37	193	C	Histone acetyltransferase
38	276	C	RecA homolog, AbiDc
39	325	S/M	Transposase	Transposase
40	65	C	Repressor protein, phage transcription regulation
41	108	S/M			+
42	452	S/M	Truncated stage III sporulation protein
43	128	C
44	428	C			+
45	435	S/M	Truncated cell division protein FtsZ	FtsZ
46	119	C			+
47	201	C	Myosin heavy chain (repeats)c
48	75	S/M
49d	227	C	Hydrolase, histone acetylasec
50	317	C			+
51	492	C	Nitrogenase, dethiobiotin synthasec
52	149	S/M	Topoisomerasec		+
53d	131	S/M	Protease, ATP dependentc		+
54d	393	S/M	S-layer protein (N terminus)	S-layer protein (N terminus)
55	790	S/M	Conserved membrane protein
56	45	S/M			+
57	142	C			+
58	272	C	RepB/ParA-nitrogenase
59	477	C	Secretory protein kinase GSPII-E	Secretory protein kinase
60	310	S/M			+
61	236	S/M			+
62	57	S/M			+
63	67	S/M			+
64	68	S/M
65d	202	S/M	Cellular recognitionc
66	361	S/M
67d	682	S/M	Cellular adhesionc
68	181	M			+
69	135	S/M
70	437	C			+
71	85	C			+
72	101	C
73	89	C
74	111	S/M			+
75	116	S/M			+
76	304	S/M	M1 peptidasec
77	91	C			+
78	405	C	Truncated TrsE homologc
79d	1,222	M	Cell wall protein, adhesinc	Hypothetical hydrophobic
80	89	S/M	Transcription regulatorc
81	424	S/M	Transposon-related NlpDc	Transposon related
82	171	C
83	127	S/M	Phage-related proteinc
84	101	S/M			+
85d	227	M	CAAX-like protease	Hypothetical
86	381	M	Transporter	Hypothetical
87	160	M	Thioreduxin isomerase	Putative thioreduxin
88	82	C	Conserved protein, transcription factor
89	158	C	Truncated RepA
90d	652	S/M	S-layer plus DNA repair, repeats	Hypothetical
91d	280	M	Phosphatidylglycerol phosphatase B	Hypothetical
92	92	S/M	N-terminus of Has-A
93	366	S/M	Has-A lacks N-terminus	Has-A
94	296	S/M	Has-C	Has-C
95	443	S/M	NDP-sugar dehydrogenase	NDP-sugar dehydrogenase
96	274	C	Transposase	Transposase
97d	150	C	BS phage SPBC2, hypotheticalc
98d	410	C	Neisseria-Clostridium imported proteinsc
99d	164	C	Ribosomal protein Zf-A20c
100	62	C
101d	170	C	DNA binding, RepAc
102	80	C
103	317	C	Integrase	Integrase
104	60	C
105d	67	C	Truncated AbrB
106	126	S/M			+
107	809	S/M	LF	LF
108	101	C	Hsp protein		+
109	99	C	PagR	PagR
110	764	S/M	PagA	PagA
111d	204	C	Domain 4 of PAc	Hypothetical protein in PA
112	288	C	GerXC	GerXC
113	492	C	GerXA	GerXA
114	359	M	GerXB	GerXB
115	193	C	Resolvase	Resolvase
116	925	S/M	Transposase	Transposase
117	132	C			+
118d	150	C	Histidine kinase (truncated)c	Unknown
119	475	S/M	AtxA	AtxA
120	190	S/M	Transposase	Transposase
121	57	S/M	Truncated Apt	Apt
122	800	S/M	EF	EF
123	37	S/M			+
124	55	C	C terminus of hemolysin II
125	125	C			+
126	151	C
127	214	S/M	Transposase	Transposase
128	84	C	Transposase
129	137	S/M	Transposase	Transposase
130d	237	S/M	Adhesin, enzyme	Hypothetical
131	349	C	Truncated GTP pyrophosphokinase
132	361	C	Integrase	Integrase
133	485	C	Truncated RECD, lectin-like foldc
134	125	C			+
135	149	C
136	364	C	Response regulator	Response regulator
137	61	S/M	Hfq	Hfq
138	97	S/M	PagR-like	PagR-like
139	96	C	Truncated DsdB	Hypothetical
140	95	S/M
141	214	S/M	Nuclease	Thermonuclease
142	887	C	Topoisomerase III	Topoisomerase
143	150	C			+

^aS/M, secretion signal or transmembrane; C, cytoplasm.

^bData from reference 44.

^cThe function assignment is partial.

^dORF discussed in the text.