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. 2002 Dec;70(12):6896–6903. doi: 10.1128/IAI.70.12.6896-6903.2002

FIG. 3.

FIG. 3.

Expression of IL-18 mRNA and protein in LPS-treated mice. Total RNA prepared from spleen and liver tissue isolated from C3H/HeN mice before and at 3 and 8 h after administration of 75 or 500 μg of LPS was analyzed by RT-PCR for IL-18 and GAPDH mRNA expression. The levels of IL-18 mRNA in the livers (A) and spleens (B) of mice treated with 75 μg (□) and 500 μg (▪) were plotted as a ratio of IL-18 mRNA to GAPDH mRNA at the time points tested. Each point represents the mean ± SE of results from three mice. (C) Equal amounts of protein from extracts of LPS-treated mouse spleens isolated at different time points were separated on 6 to 14% gradient SDS-polyacrylamide, transferred to Immobilon-P, and immunostained with anti-IL-18 antibody (1:500). Lane 1, zero hours; lanes 2, 3, and 4, at 3, 8, and 15 h after 75 μg of LPS treatment, respectively; and lanes 5, 6, and 7, at 3, 8, and 15 h after 500 μg of LPS treatment, respectively.