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. 2026 Apr 23;17:5648. doi: 10.1038/s41467-026-72203-3

Fig. 2. CAR expression of CAR-CD34+ cells transformed via lentivirus or CD34/LNP-CAR mRNA in vitro.

Fig. 2

a Representative gating strategy for flow cytometry and analyses of VCAM-1-scFv-CAR expression in freshly isolated CD34+ cells after transfection with PBS, LV-IgG-CAR, CD34/LNP, LNP-CAR mRNA, LV-VCAM-1-CAR, and CD34/LNP-CAR mRNA. b Quantitative analyses of the VCAM-1 CAR expression in the CD34+ cells between groups. Data in (b) are from n = 3 biologically independent samples. c Western blot analyses of VEGFA expression. Data in (c) are from n = 3 biologically independent samples. d The EdU assay determined the EdU-positive CD34+ cell rate. Data in (d) are from n = 5 biologically independent samples. e Flow cytometry was performed to characterize the endothelial markers (CD31 and VE-CAD) in freshly isolated CD34+ cells. Data in (e) are from n = 3 biologically independent samples. All data are presented as mean ± s.d. Statistical significance and P-values were analyzed by using one-way ANOVA followed by Tukey’s multiple comparisons test (two-sided). *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 200 µm (d); 10 µm (k). Source data are provided as a Source Data file.