Fig. 3. Functional evaluation of CAR-CD34+ cells transformed via lentivirus or CD34/LNP-CAR mRNA in vitro.
a Wound healing and transwell assays were used to evaluate CD34+ cells’ migration ability. b Quantitative data of the wound healing and transwell. Data in (b) are from n = 5 biologically independent samples. c Adhesion assays of the CD34+ cells. d Quantitative data for the adhesion assays. Data in (d) are from n = 5 biologically independent samples. e Tube formation assay of the CD34+ cells. f Tube formation was quantified by total tube formation length and branch junction. Data in (f) are from n = 5 biologically independent samples. All data are presented as mean ± s.d. Statistical significance and P-values were analyzed by using one-way ANOVA followed by Tukey’s multiple comparisons test (two-sided). *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 200 μm (a); 100 μm (c); 200 μm (e). Source data are provided as a Source Data file.
