Figure 4: Overview of RcsD dimers and structure-guided mutational analysis.
A) Multiple views of the RcsDKp Cryo-EM structure, highlighting the overall architecture. The detergent micelle has been removed for clarity. B) Refined structure of RcsDKp with detailed structural features of the protein and close-up views of the dimerization interface, showing key residues and interactions involved in dimer stabilization. C) Multiple views of the RcsDEc Cryo-EM structure, highlighting the overall architecture. The detergent micelle has been removed for clarity. D) Refined structure of RcsDEc with detailed structural features of the protein and close-up views of key residues and interactions involved in dimer stabilization. E) Signaling by RcsDEc dimer interface mutants. Strain EAW19 (ΔrcsD) was transformed with either pBAD24 vector, or RcsDEc (pEAW11) or its mutants and the fluorescence assayed in MOPS minimal glucose medium. PMBN was used for Rcs induction.