Figure 5: Overview of RcsC dimer structure and structure-guided mutational analysis.

A) Multiple views of the RcsC_Kp Cryo-EM structure, including 2D projection image, and the Cryo-EM map covering periplasmic and TM1/TM2 domains. B) Refined structure of RcsC_Kp with detailed structural features of the dimer interface. C) Multiple views of the RcsC_Ec Cryo-EM structure, including 2D projection image, and the Cryo-EM map covering periplasmic and TM1/TM2 domains. D) Refined structure of RcsC_Ec with detailed structural features of the dimer interface. E) Signaling by RcsC dimer mutants. Strain EAW91 (ΔrcsC) was transformed with either pBAD24 vector derivatives encoding the RcsC mutants and their fluorescence assayed as described in 4E.