Figure 6.

Hairpin oligonucleotides are poor substrates for TraI36-mediated cleavage. The 5′-³²P-labeled WT and G144′C 17mer (left panel) and WT and G144′C 30-base Hairpin (right panel) oligonucleotides were incubated with varying concentrations of TraI36. Oligonucleotide concentration was 4 nM, and for each reaction the TraI36 concentrations were (from left) 0 nM, 1 nM, 10 nM, 100 nM and 1 µM. After each reaction was stopped, the reaction mixtures were applied to a denaturing polyacrylamide gel. Cleavage activity is indicated by the appearance of a faster migrating band.