Table 2.

Dissociation rate constants of 3′-TAMRA-labeled ssDNA oligonucleotides from F TraI36

Oligonucleotide	Competitor	k1 (min−1)	% Amp	k2 (min−1)	% Amp	na
WT 17-base	WT 17-base	0.84 ± 0.0008	100			3
WT Hairpin	WT Hairpin	0.42 ± 0.16	27	0.026 ± 0.013	73	9
G151′C/C150′G Hairpin	WT Hairpin	0.46 ± 0.009	89	0.14 ± 0.04	11	3
G144′C Hairpin	G144′C Hairpin	0.034 ± 0.006	100			3
WT 17-base	G144′C Hairpin	0.67 ± 0.14	76	0.14 ± 0.05	24	3

^aNumber of measurements averaged.