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. 2006 Jan 17;34(2):407–416. doi: 10.1093/nar/gkj445

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© The Author 2006. Published by Oxford University Press. All rights reserved

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The purified wild-type Est3p forms dimer with gel filtration analysis. (A) Elution profile of gel filtration with an analytical size exclusion column superdex 75. The standard markers are as indicated: BSA (67 kDa), Ovalbumin (43 kDa), chymotrypsinogen A (25 kDa) and Ribonuclease A (13.7 kDa). The fractions near or within the peaks (from Fraction 3 to 14) were examined by silver staining (B) and western blot (C). Affinity-purified anti-Est3p antibody was used for western blot.