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. 2006 Jan 17;34(2):407–416. doi: 10.1093/nar/gkj445

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© The Author 2006. Published by Oxford University Press. All rights reserved

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Purification of wild-type and mutant Est3 proteins. The purified proteins were analyzed with SDS–PAGE stained by (A) Coomassie blue and (B) western blot using affinity purified polyclonal anti-Est3p antibody. (C) The over-expression of Est3 mutants in S.cerevisiae. The upper panel is a western blot probed with affinity purified polyclonal anti-Est3p antibody. The lower panel shows a western blot of the α-tubulin in the same membrane as used in the upper panel (loading control) probed with a monoclonal anti-α-tubulin antibody.