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. 2006 Jan 12;34(1):386–394. doi: 10.1093/nar/gkj433

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© The Author 2006. Published by Oxford University Press. All rights reserved

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In vitro mapping of RNA secondary structure. 100 fmol of indicated radiolabelled RNA probes of 116 nt (wt) and 109 nt (suΔU+10, suΔU+9 and suΔU−3) were incubated with oligonucleotide RH-1 complementary to the AUG regions and 0.5 U RNase H for 2 or 5 min. Major RNase H cleavage products of 59 and 30 nt (arrows) were visualized on autoradiograms after separation by denaturating PAGE. Computer-predicted secondary RNA structures with a boxed AUG initiation codon are given at the top. Position of oligonucleotide RH-1 is indicated by bold characters.