Figure 2.
Effect of siRNA dose on the duration and magnitude of luciferase knockdown by siRNA in nondividing cells. (A) Experimental results using Oligofectamine to deliver siRNA to luciferase-expressing, nondividing fibroblasts with 1.5 × 105 cells per well in a 24-well plate. Data points represent the ratio of the average luciferase signal intensity from triplicate wells receiving siGL3 and siCON1 on day 0. Squares, 10 nM; diamonds, 25 nM; triangles, 50 nM; circles, 100 nM. (B) Luciferase knockdown after siRNA transfection predicted by the mathematical model using the baseline in vitro parameters given in Table 2 with the number of cells held constant at 1.5 × 105, a transfection time of 5 h, and a transfection efficiency of 90%.