Figure 4.

Kinetics of luciferase knockdown by siRNA in Neuro2A-Luc subcutaneous tumors in A/J mice. (A) Experimental and predicted results for luciferase knockdown after three consecutive LPTV injections on days 6, 7 and 8 of transferrin-targeted CDP-Im polyplexes containing 50 µg siRNA per 20 g mouse. Experimental data points are shown for a mouse receiving siCON1 (squares) and a mouse receiving siGL3 (circles). Solid lines represent the predicted luciferase signal with siRNA treatment and dashed lines represent the predicted luciferase signal in the absence of siRNA treatment. (B) Normalization of the observed luciferase signal in the siGL3-treated mouse to the predicted luciferase signal in the absence of treatment. Circles indicate the normalized experimental data points, while the solid line represents the response predicted by the mathematical model using the baseline in vivo parameters given in Table 2 and assuming that 50% of the total cells are reached with each dose.