Figure 6.

Clinical effects of peptide injection do not occur in mice lacking TNFR1. (A) C57BL/6 or TNFR1KO mice (4 per group) were infected with LCMV and, 8 days later, were sacrificed, and their virus-specific CD8⁺ T cell responses were measured by ICCS, as described in Methods. Dot plots are gated on CD8⁺ T cells; the y axis shows IFN-γ staining, and the x axis shows TNF staining. Numbers in each of 3 quadrants indicate the CD8⁺ T cells in that quadrant as a percentage of total CD8⁺ T cells. (B) C57BL/6 or TNFR1KO mice (8 per group) were microchip-implanted, and infected with LCMV. Eight days later, the mice were injected either with peptide (6 mice per group, open symbols, dotted lines) or with saline (2 mice per group, filled symbols, solid lines), and their body temperatures were measured over the subsequent 8 hours. Crosses indicate deaths that occurred before the end of this period.