Table 2. Genomic analysis for 2 pediatric cases of acute severe hepatitis of unknown etiology associated with AAV2 and HAdV-F41 co-infection, California, USA*.
| Case no. | Source | NGS method | Virus identified | % Genome/ gene coverage | % Identity | Reference genome/gene | GenBank accession no. |
|
|---|---|---|---|---|---|---|---|---|
| Reference isolate | Patient isolate | |||||||
| 1 |
Plasma and liver | Shotgun | AAV2 | 81.3 | 95.4 | AAV isolate CHC3511_AAV.FL.circular, complete genome | MK163942 | SRX33017783 |
| Whole blood |
Amplicon (hexon gene) |
HAdV-F41 |
100 |
96.7 |
HAdV-F41 isolate 2330/N.Nov/RU/2009 hexon gene, partial cds |
HM588740 |
SRX33034982 |
|
| 2 | Plasma | Shotgun | AAV2 | 35.1 | 90.9 | AAV2, complete genome | NC_001401 | SRX33017784 |
| Whole blood | Amplicon (hexon gene) | HAdV-F41 | 86 | 99.5 | HAdV-F41isolate 2330/N.Nov/RU/2009 hexon gene, partial cds | HM588740 | SRX33034983 | |
*AAV2, adeno-associated virus type 2; HAdV-F41, human adenovirus species F type 41; cds, coding sequence; NGS, next-generation sequencing.