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. 2026 May 1;16:20217. doi: 10.1038/s41598-026-50368-7

Table 2.

Descriptive statistics (mean ± SD) of heart rate (HR) and oxygen saturation (SpO₂) across study groups and time points, with summary results of two-way repeated measures ANOVA. Abbreviations: DA, during anesthesia; DR, during rubber dam; DT, during treatment; Pre, pre-anesthesia.*Statistical analysis differences (P < 0.05). HR was analyzed using two-way repeated measures ANOVA

Jaw Variable Time Point Manual Syringe CCLAD Vibration-Assisted Jet Injection P-value
Mean ± SD
Lower Jaw HR Pre 97.93 ± 14.32 91.43 ± 16.41 91.29 ± 10.61 93.57 ± 8.20

Time effect

(P < 0.001*),

Group × Time effect

(P = 0.018*)

DA 116.93 ± 15.50 95.07 ± 18.72 102.93 ± 18.22 98.00 ± 12.69
DR 107.36 ± 16.20 91.36 ± 19.17 98.14 ± 8.98 96.57 ± 12.89
DT 99.36 ± 16.75 89.86 ± 16.19 93.00 ± 12.64 99.79 ± 15.65
SpO2 All time points ~ 98–99 ~ 98–99 ~ 98–99 ~ 98–99 > 0.05
Upper Jaw HR Pre 93.64 ± 15.68 95.07 ± 11.52 97.93 ± 8.53 93.57 ± 10.23

Time effect

(P = 0.001*),

Group × Time effect

(P = 0.264)

DA 108.71 ± 15.02 96.00 ± 11.43 110.00 ± 31.90 103.14 ± 14.87
DR 94.00 ± 15.97 96.86 ± 10.48 103.00 ± 13.40 95.71 ± 12.34
DT 97.29 ± 13.16 94.21 ± 9.47 101.71 ± 20.12 98.14 ± 9.90
SpO2 All time points ~ 98–99 ~ 98–99 ~ 98–99 ~ 98–99 > 0.05