Figure 2. (A) Northern blotting of cDNAs isolated from stage A2 ovaries of facultative apomictic guinea grass. ASG-1 is derived from A2-134 (Chen et al. 1999) and is used here as a control. ASG-2, ASG-3, ASG-4, and ASG-5 are from A2-231, A2-512, A2-133, and A2-132, respectively. At this stage (A2), AICs appear and AIC-derived embryo sacs are formed in apomictic, but not in sexual genotypes. The hybridization signals correspond to this stage and are not visible during megaspore (AS1) or sexual embryo sac formation (S2). (B) P332 is a cDNA clone found in both genotypes at stages A2 and S2, respectively and is used here as a control for clone specificity. (C) P232 is a cDNA clone found in both genotypes at stages AS1, A2, and S2 and is used here as a control for clone specificity.
