Figure 2. Mitochondrial localization quantification in human astrocytes.

(A) Per-cell estimates of mitochondrial localization show a color gradient from perinuclear (blue) to peripheral (red) mitochondria obtained from analysis of a MitoTracker-stained cell image (gray). (B) Gray layer of cells detected and mitochondria in the image. In this example, ‘Scene 14’ shows four cells. (C) Violin plot with the fraction of mitochondrial pixels (dots) identified from perinuclear (center, 0, in blue) to peripheral (periphery, 1, in red). (D) Values in the spreadsheet generated for the four cells in Panel B.