Table 7.
Robustness results of the proposed HPTLC-densitometric method.
| Parameter | Exp. Change | CFZ | MFC | SDD | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rf A | T B | K` C | Rf A | T B | K` C | Rf A | T B | K` C | ||
|
Mobile phase composition (ethyl acetate: methanol: triethylamine, v/v/v) |
50.1: 29.9: 0.9 (v/v/v) | 0.28 | 1.06 | 1.04 | 0.58 | 0.98 | 3.79 | 0.73 | 0.99 | 5.24 |
|
49.9: 30.1: 1.1 (v/v/v) |
0.29 | 1.04 | 1.01 | 0.57 | 0.97 | 3.82 | 0.71 | 1.01 | 5.26 | |
| Detection wavelength (nm) | 269 | 0.29 | 1.01 | 1.03 | 0.59 | 0.95 | 3.77 | 0.74 | 1.03 | 5.21 |
| 271 | 0.28 | 1.02 | 1.00 | 0.57 | 0.94 | 3.79 | 0.72 | 0.98 | 5.25 | |
| Scanning speed (mm/s) | 18 | 0.28 | 1.05 | 1.05 | 0.59 | 0.99 | 3.68 | 0.73 | 1.01 | 5.29 |
| 22 | 0.29 | 1.02 | 1.02 | 0.58 | 0.96 | 3.78 | 0.75 | 0.99 | 5.26 | |
| Mean | 0.29 | 1.03 | 1.03 | 0.58 | 0.97 | 3.77 | 0.73 | 1.00 | 5.25 | |
| SD | 0.01 | 0.02 | 0.02 | 0.01 | 0.02 | 0.05 | 0.01 | 0.02 | 0.03 | |
| % RSD | 1.92 | 1.90 | 1.83 | 1.54 | 1.94 | 1.27 | 1.94 | 1.83 | 0.50 | |
ARetardation factor.
BTailing factor.
CCapacity factor.