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. 2026 Jul 5;16:20571. doi: 10.1038/s41598-026-60367-3

Table 7.

Robustness results of the proposed HPTLC-densitometric method.

Parameter Exp. Change CFZ MFC SDD
Rf A T B K` C Rf A T B K` C Rf A T B K` C

Mobile phase composition

(ethyl acetate: methanol: triethylamine, v/v/v)

50.1: 29.9: 0.9 (v/v/v) 0.28 1.06 1.04 0.58 0.98 3.79 0.73 0.99 5.24

49.9: 30.1: 1.1

(v/v/v)

0.29 1.04 1.01 0.57 0.97 3.82 0.71 1.01 5.26
Detection wavelength (nm) 269 0.29 1.01 1.03 0.59 0.95 3.77 0.74 1.03 5.21
271 0.28 1.02 1.00 0.57 0.94 3.79 0.72 0.98 5.25
Scanning speed (mm/s) 18 0.28 1.05 1.05 0.59 0.99 3.68 0.73 1.01 5.29
22 0.29 1.02 1.02 0.58 0.96 3.78 0.75 0.99 5.26
Mean 0.29 1.03 1.03 0.58 0.97 3.77 0.73 1.00 5.25
SD 0.01 0.02 0.02 0.01 0.02 0.05 0.01 0.02 0.03
% RSD 1.92 1.90 1.83 1.54 1.94 1.27 1.94 1.83 0.50

ARetardation factor.

BTailing factor.

CCapacity factor.