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. 2026 Jun 22;82(Pt 7):245–251. doi: 10.1107/S2053230X26006333

Table 2. Crystallization.

Method Vapor diffusion, sitting drop
Plate type 96-well plate, MRC
Temperature (K) 277
Protein concentration (mg ml−1) 2.0
Buffer composition of protein solution 20 mM HEPES–NaOH pH 7.5, 0.2 M NaCl
Composition of reservoir solution 0.2 M ammonium nitrate, 20%(w/v) PEG 3350
Volume and ratio of drop 1.6 µl, 0.8:0.8 (protein:reservoir)
Volume of reservoir (µl) 30