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. 2002 Oct 24;3(11):research0062.1–research0062.12. doi: 10.1186/gb-2002-3-11-research0062

Figure 1.

Figure 1

Self-self hybridization of the KM12L4A cell line. (a) R-I (ratio-intensity) plot for a self-self hybridization of the KM12L4A cell line before lowess correction. (b) The same dataset, showing the effect of lowess correction (red) relative to the uncorrected data (blue). Lowess removes the intensity-dependent curvature that is evident in the uncorrected data and in the process, reduces the SD in the dataset. (c) Similar plots for all 30 self-self hybridizations performed in this study, including the SD for the dataset before (blue) and after (red) the application of the lowess correction.