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. 2006 Jan 9;103(3):696–701. doi: 10.1073/pnas.0504187103

Fig. 5.

Fig. 5.

CB2-specific agonist, HU-308, restrains osteoclastogenesis. (A) Number of TRAP-positive multinucleated cells in primary bone marrow-derived monocyte culture from WT (filled squares) and CB2–/– (open squares) mice maintained for 6 days in osteoclastogenic conditions (M-CSF and RANKL) with the indicated HU-308 concentrations. Cntl, control without HU-308. Data are mean ± SE obtained in 24 culture wells per condition. (B) Number of TRAP-positive multinucleated cells in RAW 264.7 cell cultures incubated for 7 days with RANKL and the indicated HU-308 concentrations. (C) DNA synthesis in RAW 264.7 cultured for 3 days with (open circles) or without (filled circles) RANKL and HU-308 as indicated. Data in B and C are mean ± SE obtained in triplicate culture wells per condition. (D) RT-PCR analysis of RANKL and osteoprotegerin (OPG) expression in bone marrow-derived primary stromal cell cultures. Cells from WT mice were incubated for 10 days in osteogenic medium and then challenged for 8 h with HU-308 or control (Cntl) medium. *, P < 0.05.