Fig. 3.

Injection of Xbves morpholino into a two-cell X. laevis embryos inhibits gastrulation movements. (A, C, E, and G) Uninjected embryos serve as a control. Arrows indicate the blastopore (D) and the forming neural plate (E, G, and H). At stage 6, Xbves MO-injected embryos develop in an apparently normal fashion (B). By stage 11, experimental embryos are delayed or inhibited in yolk plug closure (D, arrow denotes the edge of the yolk plug), and by stage 14 (F), most embryos lack closed yolk plugs and have become necrotic. Control sense MO-injected embryos (H) complete gastrulation and undergo neurulation (neural plate, white arrows) at the same rate compared with uninjected embryos (G).