Table 1. AGS cDNAs isolated in functional screens for receptor-independent AGS.
| Tissue used to generate cDNA libraries for functional screen*
|
||||
|---|---|---|---|---|
| AGS | Protein or gene names in databases and literature | Repetitive transient cardiac ischemia (rat) | Heart (human) | Prostate Leiomyosarcoma (human) |
| AGS1 | DexRas1, RASD1 | + | - | - |
| AGS3 | GPSM1 (C-terminal segment with 3-4 GPR motifs) | + | + | + |
| AGS4 | G18.1b, GPSM3 (three GPR motifs) | + | - | + |
| AGS5 | LGN, GPSM2 (C-terminal 322 amino acids, four GPR motifs) | - | - | + |
| AGS6 | RGS12 (C-terminal 365 amino acids, one GPR motif) | - | - | + |
| AGS7 | TRIP13† | - | - | + |
| AGS8 | KIAA 1866 (C-terminal 372 amino acids) | + | - | - |
| AGS9 | Rpn10 | + | + | - |
| AGS10 | Gαo | + | - | - |
AGS refers to cDNAs isolated in the functional screen and they are numbered according to the order in which they were isolated. Unless indicated otherwise, isolated cDNAs encoded the entire coding sequence. GPR, G protein regulatory motif (13). The rat heart cDNA library contained 3.1 × 1011 colony-forming units (cfu) with an average insert size of 2.0 kb. The human heart cDNA library contained 2.4 × 107 with an average insert size of 1.6 kb. The human prostate leiomyosarcoma cDNA library contained 1.5 × 107 cfu with average insert size of 1.3 kb. Number of transformants screened for each library: Repetitive transient ischemic heart (rat), 3.1 × 105; prostate leiomyosarcoma (human), 4.3 × 106; normal heart (human), 1.0 × 106.
cDNA libraries were screened in yeast strains CY1141 (Gαi3), CY8342 (Gαs), and CY9603 (Gα16) (12, 13).
C-terminal sequence, unpublished data.