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. 2026 Jun 22;98(26):19603–19614. doi: 10.1021/acs.analchem.6c01280

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© 2026 The Authors. Published by American Chemical Society

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Multiomics of sEVs isolated from MDA-MB-231 cells. Multiomics analysis was performed in triplicates (n = 3). Average number of (A) peptides and (B) protein groups identified from sEVs. (C) Overlap between proteins identified in sEVs isolated from conditioned cell culture media (pink) with databases of extracellular vesicle proteins (Vesiclepedia; green) and small extracellular vesicle proteins (ExoCarta; blue). (D) Average number of features found in the organic fraction (yellow) and aqueous fraction (blue). (E) Average number of annotated features in the organic fraction (yellow) and aqueous fraction (blue). (F) Lipid class distribution identified in the organic fraction. PC: phosphatidylcholines; PE: phosphatidylethanolamines; PS: phosphatidylserines; SM: sphingomyelins; HexCer: hexosylceramides; and LPE: lysophosphatidylethanolamines.