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. 2026 Jun 22;98(26):19603–19614. doi: 10.1021/acs.analchem.6c01280

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© 2026 The Authors. Published by American Chemical Society

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Comparative proteomics indicates differences in the purity of sEV preparation approaches. Five replicates (n = 5) were analyzed per condition. (A) Volcano plot illustrating proteomic differences between sEV populations isolated by ultracentrifugation (UC, green) and polymer precipitation (PPT, red). (B) Gene Ontology Subcellular Localization (GOSCL) analysis of proteins that are significantly more abundant in PPT compared to UC. (C) Volcano plot illustrating proteomic differences in sEV populations isolated using UC (green) and size exclusion chromatography with ultrafiltration (SECUF, blue). (D) GOSCL analysis of proteins that are significantly more abundant in SECUF proteomes compared to UC. (E) Box-and-whisker plots showing the differences in the abundances of lipoprotein-associated proteins across purification conditions. (F) Box-and-whisker plots showing the differences in the abundances of canonical sEV biomarker proteins across purification approaches.