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. 2026 Jun 22;98(26):19603–19614. doi: 10.1021/acs.analchem.6c01280

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© 2026 The Authors. Published by American Chemical Society

This article is licensed under CC-BY 4.0

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Lipidomics analysis of sEVs from plasma reveals population differences associated with purification approach. Five replicates (n = 5) were analyzed per condition. Average number of (A) features and (B) annotated features identified through analysis of the organic fraction of extracted sEVs purified using ultracentrifugation (UC), size exclusion chromatography with ultrafiltration (SECUF), and polymer precipitation (PPT). (C) Principal component analysis and (D) hierarchical clustering illustrating the dissimilarity among the different purification approaches. (E) Stacked bar plot illustrating the relative lipid class composition of annotated features in the sEV lipidomes across purification approaches. Cer: ceramides; FA: fatty acids; HexCer: hexosylceramides; LPC: lysophosphatidylcholines; PC: phosphatidylcholines; PE: phosphatidylethanolamines; PI: phosphatidylinositols; SM: sphingomyelins; and TG: triacylglycerols.