7.

Metabolomic coverage and composition depend on the purification approach. Five replicates (n = 5) were analyzed per condition. Average number of (A) features and (B) annotated features identified using ultracentrifugation (UC), size exclusion chromatography with ultrafiltration (SECUF), and polymer precipitation (PPT). (C) Principal component analysis illustrating differences in the metabolome among the different purification approaches. (D) Hierarchical clustering analysis showing the difference across purification methods. (E) UpSet plot displaying the degree of overlap in identified features among the different conditions.