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. 2001 Feb 1;20(3):330–339. doi: 10.1093/emboj/20.3.330

graphic file with name cde052f1.jpg

Fig. 1. Distinct targeting patterns displayed by ACP–GFP, ROP1–GFP and ACP–GFP–mROP1. Fusion of the nuclear-encoded apicoplast protein ACP to GFP targets this recombinant reporter molecule into the apicoplast in stable transgenic parasites (A). Similarly, stable expression of the rhoptry protein ROP1 fused to GFP results in targeting to the rhoptries in every parasite (B). Transient expression of the ACP–GFP–mROP1 construct reveals a ring-like staining pattern in only one parasite per vacuole (C). All panels show superimposed fluorescence and phase-contrast images of living parasites. Bar = 5 µm.