Table I. Dose of NUP98–HOXA9-transduced cells transplanted per mouse.
Virus [number of mice] | ID #, symbola | No. of CFCb transplanted per mouse |
No. of infected LTRCc transplanted per mouse | |
---|---|---|---|---|
Total | Transducedd | |||
NUP98-HOXA9 (Neo) [4] | 1–4, cross | 1800 | 144 | 1 |
NUP98-HOXA9 (EGFP) [7] | 5–11, cross | 1750 | 350 | 4 |
NUP98-HOXA9 (EGFP) [5] | 12–16, circle | 31 100 | 19 300 | 193 |
NUP98-HOXA9 (EGFP) [2] | 17, 18, diamond | 11 400 | 6640 | 66 |
EGFP-1e [4] | – | 1150 | 259 | 3 |
EGFP-2 [3] | – | 54 000 | 36 600 | 366 |
EGFP-3 [5] | – | 22 900 | 8470 | 85 |
aSymbols are used to represent mice from each group in Figure 4C.
bMyeloid colony forming cells, as measured in clonogenic progenitor assays.
cEstimated: reported to be ∼1% of transduced CFC frequency (Sauvageau et al., 1995; Thorsteinsdottir et al., 1999).
dAs determined by resistance to G-418 (Neo viruses), or detection of fluorescence (EGFP viruses) either by FACS or microscopy.
eThe group of EGFP mice that most resembled NUP98–HOXA9 mice with respect to transplanted dose of transduced CFC was used when needed as controls.