Fig. 8. Hsp72 suppresses the transactivating activity of c-Jun stimulated by the JNK pathway. NIH 3T3 cells were transiently transfected with a luciferase reporter plasmid pFR-Luc, pFA2-c-jun, pFC-MEKK1, pCMV-Hsp72 or pcDNA3-SEK1(K129R) as indicated. pcDNA3-β-gal was included in all transfections. After 50 h, the transfectants were lysed and assayed for luciferase activity. Luciferase reporter activity in each sample was normalized according to the β-galactosidase activity measured in the same sample.