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. 2026 May 19;16:354. doi: 10.1038/s41398-026-04096-1

Table 4.

Association between cumulative exposure to cannabis and hippocampal volume, fractional anisotropy (FA) and cerebral blood flow (CBF) at Year 30, stratified by sex, multivariable adjusted.

Variable NEVER TOBACCO SMOKING
Standardized difference in MRI measures (95% CI)a
EVER TOBACCO SMOKING
Standardized difference in MRI measures (95% CI)a
Women Men Women Men
Hippocampus volume (mm3)
-Never user Reference Reference Reference Reference
-1 day to 0.5 cannabis years −25.66 (−122.13–70.82) −137.17 (−304.84–30.49) −160.03 (−346.02–25.96) 18.35 (−341.58–378.29)
-0.5 to < 2 cannabis years 57.60 (−77.46–192.65) −158.19 (−356.04–39.66) −174.60 (−371.99–22.79) 31.04 (−326.35–388.42)
->2 cannabis years −21.13 (−209.69–167.43) −31.57 (−312.55–249.40) −124.94 (−341.89–92.01) −94.22 (−449.17–260.73)
P-value for trenda/ R2 0.5/ 0.5 0.3/ 0.4 0.3/ 0.51 0.4/ 0.45
Fornix FA
-Never user Reference Reference Reference Reference
-1 day to 0.5 cannabis years 0.0005 (−0.0125–0.0134) −0.0084 (−0.0247–0.0079) 0.0059 (−0.0153–0.0272) 0.0137 (−0.0252–0.0526)
->2 cannabis years −0.0005 (−0.0253–0.0242) −0.0090 (−0.0362–0.0182) 0.0091 (−0.0156–0.0338) 0.0154 (−0.0229–0.0537)
P-value for trendb/ R2 0.6/ 0.22 0.7/ 0.15 0.5/ 0.31 0.7/ 0.27
Hippocampus CBF (ml/100 g/min)
-Never user Reference Reference Reference Reference
-1 day to 0.5 cannabis years 1.26 (−4.82–7.34) −0.98 (−6.11–4.16) 7.27 (0.60–13.93) −4.15 (−16.87–8.57)
-0.5 to < 2 cannabis years −2.28 (−10.74–6.17) −1.54 (−7.98–4.90) 5.08 (−1.84–11.99) −5.70 (−18.21–6.82)
->2 cannabis years 2.06 (−7.91–12.03) −4.18 (−12.50 to) 9.96 (1.62–18.29) −0.96 (−13.33–11.42)
P-value for trendb/ R2 0.7/ 0.13 0.8/ 0.28 0.09/ 0.45 0.3/ 0.48

648 CARDIA participants at the Year 30 MRI sub-study excluding participants with a history of stroke or TIA.

Stratified by ever tobacco smoking.

FA fractional anisotropy, CBF cerebral blood flow, CARDIA coronary artery risk development in young adults study, N number of participants; 95% CI 95% confidence interval.

aP-values from a Wald test. All P-values two sided.