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. Author manuscript; available in PMC: 2026 Jul 11.
Published in final edited form as: Nat Rev Methods Primers. 2025 Aug 7;5:52. doi: 10.1038/s43586-025-00421-y

Table 3:

Probes integrating FEx-compatible functional groups designed to profile various proteins at specific residues.

Reference Cell Line/
Tissue		 Cell
Mode		 Enrichment
Method		 FEx Functional
Group (Number of
Molecules)		 Example Probe Structure Protein Identified
Cravatt 1999206 Rat tissues Lysate Direct (Biotin tag) Fluorophosphate (1) graphic file with name nihms-2181599-t0020.jpg FAAH
van der Hoorn and Weerapana 2012204 HeLa (spike) Lysate Direct (Alkyne tag) Sulfonyl fluoride (1) graphic file with name nihms-2181599-t0021.jpg Chymotrypsin
van der Hoorn 2013207 Mouse liver and pancreas Lysate Direct (Alkyne tag) Sulfonyl fluoride (1) graphic file with name nihms-2181599-t0022.jpg GST
Sharpless and Kelly 2016208 HeLa Lysate Direct (Alkyne tag) Fluorosulfate (3) graphic file with name nihms-2181599-t0023.jpg CRABP2 FABP
Taunton 2017209 Jurkat Intact cell Direct (Alkyne tag) Sulfonyl fluoride (3) graphic file with name nihms-2181599-t0024.jpg 133 kinases
Sharpless, Wilson and Kelly 2018203 HEK293T Lysate Competition Fluorosulfate (6) graphic file with name nihms-2181599-t0025.jpg GSTO1
Sharpless and Kelly 2020210 HEK293T Lysate Direct (Alkyne tag) Sulfuramidimidoyl fluorides (16) graphic file with name nihms-2181599-t0026.jpg PARP1
Abbasov and Cravatt 2021211 MDA-MB-231 Lysate Competition (Lysine-directed) Sulfonyl fluoride (20) graphic file with name nihms-2181599-t0027.jpg CPOX
Burley and Bush 2023212 Jurkat Intact cells Direct (Alkyne tag) Fluorosulfate (1) graphic file with name nihms-2181599-t0028.jpg CDK
Sulfonyl fluoride (6) graphic file with name nihms-2181599-t0029.jpg CDK
Sulfamoyl fluoride (1) graphic file with name nihms-2181599-t0030.jpg PIKK
Taunton 2023213 Jurkat Intact cells Direct (Alkyne tag) Sulfonyl fluoride (20, 10x Enantiomers) graphic file with name nihms-2181599-t0031.jpg APMAP

FAAH: fatty acid amide hydrolase. GST: glutathione transferases. FABP: fatty acid binding protein. CRABP: cellular retinoic acid binding protein. GSTO1: glutathione S-transferase omega 1. PARP1: poly ADP-ribose polymerase 1. CPOX: coproporphyrinogen oxidase. CDK: cyclin dependent kinase. PIKK: phosphatidylinositol 3 kinase related protein kinase. APMAP: adipocyte plasma membrane-associated protein.