FIG. 1.

Targeting of the paxillin locus and generation of the null mutation. (A) The targeting vector used to generate the null mutation is diagrammed. SA, adenovirus splice acceptor; βgeo, β-galactosidase-neomycin resistance gene fusion; pGK, phosphoglucokinase promoter; DTA, diphtheria toxin A subunit; bPA, bovine(poly A). Primers used for genotyping are diagrammed (a, b, and c). Exons are represented by black boxes. Exons 2 and 3 were deleted and encode amino acids 5 to 74. Restriction sites used to generate the targeting vector are indicated. RI, EcoRI; H_II, HindII; H_III, HindIII (see Materials and Methods for more detail). (B) Loss of paxillin expression in paxillin^−/− cells. Lysates prepared from wild-type, paxillin^+/−, or paxillin^−/− embryos were subjected to SDS-PAGE, immobilized on nitrocellulose, and immunoblotted with an antibody to paxillin.