FIG. 5.

Sur7p and its homologues function in sporulation. Strains homozygous for sur7Δ, SUR7-GFP, ydl222Δ, YDL222-3HA, ynl194Δ, or YNL194-GFP (YJC2661, YJC2210, YJC2632, YJC2638, YJC2204, YJC2629) were sporulated on an MSPO plate for 7 days at 30°C. Well-formed asci with three to four clearly visible spores, as well as incomplete asci containing only two spores, were counted. Percentages shown are of the total number of cells and asci. Error bars show the standard error of the mean for 4 to 13 independent experiments, each of which counted 500 to 2,000 cells. Data from each experiment were normalized to results for a wild-type strain (YJC1411) from the same plate, in which 4 to 10% of cells became well-formed asci.