FIG. 7.

The 29-dG oligonucleotide does not inhibit abortive initiation. (A) Schematic of the abortive-initiation assay, a steady-state assay monitoring production of 3-nt RNAs. (B) Abortive initiation was performed on the AdMLP in the absence and presence of the 29-dG oligonucleotide (250 nM). When present, the 29-dG oligonucleotide was added at point 2 (see Fig. 3A). ApCpU product was quantitated at different time points after addition of nucleotides. Each error bar represents 1 standard deviation.