FIG. 1.

Expression of DR5 and TRAIL by human astroglioma cells. (A) FACS analysis of DR5 expression on CRT-MG and U87-MG cells was performed as described in Materials and Methods. Thin lines indicate negative controls stained with secondary antibody conjugated to PE after incubation with an isotype-matched (IgG1) control antibody. (B) Cell lysates were prepared from CRT-MG and U87-MG cells, and 100 μg of protein was electrophoresed in 10% sodium dodecyl sulfate gels. Western blot analysis for TRAIL protein expression was done as described in Materials and Methods. The data are representative of three independent experiments.