Reagents and Tools Table

Reagent/resource	Reference or source	Identifier or catalog number
Experimental models
U-2 OS (U2OS)	ATCC	HTB-96
DLD-1 (DLD1)	ATCC	CCL-221
DLD1 CIP2A KO clone 7	Dan Durocher	Adam et al, 2021
DLD1 CIP2A KO clone 37	Dan Durocher	Adam et al, 2021
DLD1 + TOPBP1 BRCT6 FKBP12 (B6L)	Dan Durocher	Adam et al, 2021
DLD1 BRCA2 KO	ATCC	CVCL_HD57
hTERT RPE-1 (RPE-1)	ATCC	CRL-4000
RPE-1 CIP2A KO	This lab	De Marco Zompit et al, 2022
RPE-1 p53 KO	Dan Durocher	Adam et al, 2021
RPE-1 p53 KO CIP2A KO	Dan Durocher	Adam et al, 2021
U2OS RAD52-YFP	Jiri Lukas	Bekker-Jensen et al, 2006
CAPAN-1	Simon Powell	Xia et al, 2001
CAPAN-1 + BRCA2	Simon Powell	Xia et al, 2001
Recombinant DNA
Antibodies
Anti-CIP2A	Santa Cruz	sc-80659, RRID:AB_1121640
Anti-TOPBP1	Millipore	ABE1463,
Anti-CENPA	Abcam	ab13939, RRID:AB_300766
Anti-pSer10 Histone H3	Millipore	06-570, RRID:AB_310177
Anti-MUS81	Santa Cruz	sc-53382, RRID:AB_2147138
Anti-FANCD2	Novus Biologicals	NB100-182, RRID:AB_10002867
Anti-SLX4	John Rouse	Wilson et al, 2013
Anti-BTBD12 (SLX4)	Bethyl	A302-207A, RRID:AB_1850156
Anti-XPF	Abcam	ab76948, RRID:AB_1524575
Anti-RAD52	Santa Cruz	sc-365341, RRID:AB_10851346
Anti-p53	Abcam	ab32389, RRID:AB_776981
Anti-Tubulin	Bio-Rad	12004166
GFP-Booster Alexa Fluor® 488	Proteintech	gb2AF488-10
Oligonucleotides and other sequence-based reagents
siCTRL	Microsynth AG	UGGUUUACAUGUCGACUAA-dTdT
siCIP2A	Human KIAA1524 siRNA SMARTpool	L-014135-01-0005
Chemicals, enzymes and other reagents
Aphidicolin	MedChem Express	HY-N6733
RO-3306	SelleckChem	S7747
Karyomax Colcemid	Gibco	1521201
VE-821 ATR inhibitor	SelleckChem	S8007
Shield-1	Takara Bio	632189
Click-iT™ EdU Cell Proliferation Kit	Thermo Fisher	C10337
Duolink® PLA Reagents	Sigma-Aldrich	DUO92001, DUO92005, DUO92008, DUO82049
Software
CellProfiler 4.2.8	www.cellprofiler.org	Stirling et al, 2021
GraphPad Prism 11.0.0 (93)	www.graphpad.com
Fiji	https://imagej.net/software/fiji/	Schindelin et al, 2012