FIG. 2.
Coordinated accumulation of Aurora kinases and H3 phosphorylation during the G2/M transition. (a) Western blot analysis using anti-Aurora-B-Cter and anti-Aurora-B-Nter on 10 μg of total-protein extracts prepared from HeLa cells nontransfected, transfected with myc-tagged Aurora-A, Aurora-B, or Aurora-C, and arrested in G1/S or 10 h after release. (b) Total-protein extracts (10 μg) prepared from synchronized HeLa and NIH 3T3 cells harvested at the indicated times were analyzed by Western blot analysis using anti-Aurora-A, anti-Aurora-B-Cter, anti-Aurora-B-Nter, and anti-P.H3.