FIG. 5.

Localization of Aurora-A and Aurora-B in relation to that of P.H3 in HeLa and NIH 3T3 cells during the G₂, phase, prophase, and metaphase. NIH 3T3 cells in G₂ (top row) and HeLa cells in G₂, prophase, and metaphase (bottom three rows) were stained with anti-P.H3 in combination either with anti-IAK-1, to identify Aurora-A (left panels), or with anti-Aurora-BNter, to identify Aurora-B (right panels). The cell cycle phases were identified by the DAPI staining. Double labeling is indicated as a merge. The whole stack of images for each individual panel was subsequentely rotated by 90° on the axis (see arrow) to produce a Z-section (panel “rotated images”).