FIG. 6.

Interaction between the N-terminal tail of histone H3 and Aurora-A and Aurora-B. (a) Different susceptibilities of Aurora-A, Aurora-B, and P.H3 to increasing concentrations of salt. Protein extracts were prepared from HeLa cells using buffers with 100 and 600 mM salt concentration and analyzed by Western blotting with anti-IAK-1 to identify Aurora-A (top panel) or with anti-Aurora-B-Nter to identify Aurora-B (bottom panel). (b) Aurora-A and Aurora-B interact with the histone H3 tail. Total-protein extracts from synchronous HeLa cells were incubated with GST alone or GST-H3, and the pulled-down products were tested by Western blotting with anti-Aurora-A (top panel) and anti-Aurora-B-Nter (bottom panel) antibodies. (c) Aurora-A and Aurora-B interact with the histone H4 tail less efficiently than with the H3 tail. Equal amounts of GST fusions (not shown) were verified by Coomassie staining.