FIG. 8.

Kinase activity of the recombinant bacterial Aurora kinases. (a) Western blot analysis performed on the recombinant bacterial Aurora kinases using the anti-His antibody. (b to d) In vitro kinase assay performed with recombinant bacterial Aurora-A, Aurora-B, and Aurora-C and protein kinase A on MBP (b), free histones (c), and nucleosomes (d). The presence of equal amounts of histones in each reaction was verified by Coomassie staining. (e) In vitro kinase assay performed with recombinant bacterial Aurora-A analyzed by Western blotting. (f) In vitro kinase assay performed with recombinant bacterial Aurora-A on wild-type and mutated (S10A) histone H3 peptides (aa 1 to 30).