FIG. 4.

Srb10 is not required for Tup1 recruitment to target loci. Chromatin immunoprecipitations (IP) were performed on cell extracts from wild-type (WT) and srb10 strains containing HA-TUP1 by using an antibody against HA. Primer sets spanning both STE6 and RNR2 were used. The location of the PCR primer sets is as in Fig. 1A for STE6 and Fig. 2B for RNR2. Quantitative PCR products from one representative experiment are shown for position −0.2 kb of STE6 (A) and position −0.4 kb of RNR2 (C). Data for all primer sets are graphed for STE6 (B) and RNR2 (D). For STE6, the bar graph represents the ratio between the signals from α cells and a cells as well as the ratio between the signals from srb10 α cells and srb10 a cells. For RNR2, the bar graph represents the ratio between the signals from WT cells and crt1 cells as well as the ratio between signals from srb10 cells and crt1 cells. For the graph, three independent experiments were averaged and the error bars are shown.