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Effects of the deacetylase inhibitor TSA on cyclin E mRNA levels in RB+/+ and RB−/− MEFs. TSA was added to G0-arrested cells for the indicated times before lysis and RNA purification. RT-PCR was performed in order to visualize the small amounts of cyclin E mRNA present in arrested RB+/+ cells. The primers used were from either cyclin E or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA sequences.
