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ChIP analysis of pRb and HDAC1 binding to the mouse cyclin E promoter in RB+/+ and RB−/− MEFs. Cells were arrested in G0 (−serum) or induced to late G1 (+serum) prior to harvest. Lysates were digested with micrococcal nuclease and immunoprecipitated using anti-pRb or anti-HDAC1 antibody. Primers amplified the region of the cyclin E promoter from +46 to −47 (position +1 and primer set 12). Cntl, control; IP, immunoprecipitation.
