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. 2002 Feb;22(3):849–855. doi: 10.1128/MCB.22.3.849-855.2002

FIG. 2.

FIG. 2.

ALDH3a1 expression. (A) SDS-PAGE (10% polyacrylamide) of 10 μg of water-soluble protein from the corneal epithelium of ALDH3a1 wild-type (+/+), heterozygote (+/−), and knockout (−/−) mice. The size and position of markers are shown on the left. The major 68-kDa band is transketolase (38), and the major 51-kDa band is ALDH3a1. (B) Immunoblot probed with anti-ALDH3 antibody. SDS-PAGE was performed as in panel A, except that 1 μg of protein was loaded in each lane. (C) Zymogram for ALDH3a1 activity using benzaldehyde and NADP+ as substrates. SDS-PAGE was performed as in Panel A.