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Immunoblot analysis to test for compensation by ALDH1. (A) For SDS-PAGE, 10 μg of water-soluble protein from wild-type (+/+) and knockout (−/−) corneal epithelia were loaded onto each lane; after SDS-PAGE, the blots were stained with SyproRuby to reveal proteins. (B) Immunoblot probed with anti-ALDH1 antibody.
