Sequence alignment of Arabidopsis AtFUT proteins with α-1,6-fucosyltransferases from bacteria shows peptide motifs present in the AtFUT family. A, Region strongly conserved between family members. B, Motif shared by α-1,2- and α-1,6-fucosyltransferases hypothesized to bind GDP-Fuc. The shaded Arg (R) was shown to be necessary for human FUT1 activity in vitro. C, Combination of motif III from α-1,6-glycosyltransferases and motif III from α-1,2-glycosyltransferases that is conserved in the AtFUT family. GenBank accession numbers: AF417473, AtFUT3; AF417474, AtFUT4; AF417475, AtFUT5; AC005313, AtFUT2 (At2g03210); AC006920, AtFUT10 (At2g15350); AC007576, AtFUT6 (At1g14080), AtFUT7 (F7A19.15), AtFUT8 (At1g14100), and AtFUT9 (At1g14110). GenBank protein ID numbers: g1293900, Azorhizobium caulinodans NodZ; g404790, Bradyrhizobium japonicum NodZ; g3347912, Sinorhizobium fredii NodZ; and g5231145, AtFUT1.