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. 2001 Dec;127(4):1626–1634.

Figure 6.

Figure 6

In vitro processing of RD21. A, The recombinant proRD21 was incubated alone at pH 8.0 (lane 1) or pH 5.5 (lane 2) for 16 h, and was then subjected to SDS-PAGE followed by immunoblot analysis with anti-RD21 antibodies. B, iRD21 was extracted from 2-d-old Arabidopsis seedlings. iRD21 was incubated at pH 8.0 (lane 1) or pH 5.5 (lane 2) for 16 h, and was then subjected to SDS-PAGE followed by immunoblot analysis. C, The extract from the Arabidopsis leaves was prepared and used as a processing enzyme source. The recombinant proRD21 was incubated with the leaf extract at pH 5.5 for 16 h, and was then subjected to SDS-PAGE followed by immunoblot analysis (lane 2). The recombinant proRD21 (lane 1) and the leaf extract used as an enzyme source (lane 3) are also shown on the blot. rp, Recombinant proRD21; i, iRD21; m, mRD21. The molecular mass of each marker protein is given on the left in kilodaltons.