Figure 4.

GUS expression patterns in transverse sections of stem tissue from transgenic Arabidopsis plants. A, Transverse sections from Col-0 plants transformed with empty vector, 35S:GUS, RTM1:GUS, or RTM2:GUS were incubated in the histochemical substrate, X-gluc, at 37°C for 30 min. Sections were cleared in 75% (v/v) ethanol at 70°C. B, Higher magnification of vascular bundles in sections of RTM1:GUS and RTM2:GUS transgenic plants. C, Cortex; En, endodermis; Ph, phloem; X, xylem; Pi, pith. Scale bar = 100 μm.